Your grant proposal will be based on a hypothetical experimental observation where you have noted a change in the observable phenotype of a cell model: Molecular Cell Biology Research Paper, UOY, UK

Your grant proposal will be based on a hypothetical experimental observation where you have noted a change in the observable phenotype of a cell model, which you attribute to a change in a specific gene.

This may include a disease model. You then need to formulate the course of action required to determine which protein-coding gene is altered, as well as the mechanism of action of the protein product.

You will need to select three different methods to be used in sequence, describe each of these methods, justify your choice of approach, as well as note the potential limitations of your choice of methods.

You will need to select a relevant eukaryotic cell model (eg. yeast, cells grown in tissue culture), a specific cell function of your choice (eg. protein trafficking, cell signalling, cell cycle regulation, cytoskeleton, or other), and a particular observable phenotype (eg. change in structure or function of the cell, change in protein levels on a gel etc) of your choice.