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Outline the setup of a triple quadrupole instrument for the identification and quantification of a drug of known m/z: Advanced Analytical Science Assignment, SHU, UK

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Outline the setup of a triple quadrupole instrument for the identification and quantification of a drug of known m/z: Advanced Analytical Science Assignment, SHU, UK

UniversitySheffield Hallam University (SHU)
SubjectAdvanced Analytical Science
  • Outline the setup of a triple quadrupole instrument for the identification and quantification of a drug of known m/z from liver tissue homogenate that loses a water molecule when subjected to MS/MS analysis.
  • Explain the benefits of using derivatization in compound-specific IRMS and some of the issues encountered with this approach.
  • Biopharmaceuticals such as monoclonal antibodies and drug conjugates require careful quality control. Use your knowledge of bioanalytical chemistry to state with a short explanation an approach that could be used to determine the accurate mass of a biopharmaceutical.
  • Why is high mass resolution important in label-free quantitative proteomics carried out using “bottom-up” techniques?

 

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